Fire Rescue Victoria (FRV) firefighters responded to a grass and scrub fire along the Merri Concourse, Campbellfield, after several calls to triple-0 about 11:11pm last night.
Firefighters, assisted by CFA, attacked a 400 metre fire travelling south east toward the Hume Freeway.
Thirty-eight FRV and CFA appliances attended the scene with the fire deemed under control at 12:18am.
The response was escalated to a third alarm with crews attacking the fire from two sectors with one northbound lane of the Hume Freeway closed for the safety of fire crews. It has since re-opened.
Local councils have been requested to attend today as well as an arborist, to assess numerous trees impacted by fire on both sides of Merri Creek.
Some trees remain alight in the northern sector of the fire and will be assessed during daylight.
The scene was handed to Victoria Police at 2:20am.
The fire has been deemed suspicious.